


Depending on the images you are able to achieve already this may be sufficient this is for sure worth a try and may be the shortest way to the desired results. You may also have a look on deconvolution. Could you provide me some more information on the Fluorophore you are using, the wavelength set for excitation and the temporal resolution you would like to achieve? There is a convenient APP called Microscope Resolution from Andrew Barlow which allows you to get a good idea of the numbers. In principle the achievable resolution with 2P excitation is lower than with a 1P approach due to the longer wavelength used and the values you state (50-150nm) are below what you would expect to easily resolve. PubMed PMID: 15935220.ĭear Ali, from a purist resolution approach this will be challenging and depends on where in the sample (depth/density/labelling density and signal strength) you would like to make your observation. Microglial cells in the ischemic rat brain using principal component analysis. PubMed PMID: 23386810 PubMed Centralĥ: Soltys Z, Orzylowska-Sliwinska O, Zaremba M, Orlowski D, Piechota M,įiedorowicz A, Janeczko K, Oderfeld-Nowak B. Quantitating the subtleties of microglial PubMed PMID: 25927064 PubMed CentralĤ: Karperien A, Ahammer H, Jelinek HF. Fractal, multifractal, and lacunarity analysis of
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